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Deploy Django on Google App Engine Standard with Cloud SQL PostgreSQL. Covers Unix socket connections, Cloud SQL Auth Proxy for local dev, Gunicorn configuration, and production-ready settings. Use when: deploying Django to App Engine, configuring C...
Detect sample contamination and cross-species reads using FastQ Screen. Screen reads against multiple reference genomes to identify bacterial, viral, adapter, or sample swap contamination. Use when suspecting cross-contamination or working with sampl...
Analyze population structure using PCA and admixture analysis with PLINK and ADMIXTURE. Identify population clusters, assess ancestry proportions, visualize genetic structure, and choose optimal K for admixture models. Use when analyzing population s...
Enumerates chemical libraries through reaction SMARTS transformations using RDKit. Generates virtual compound libraries from building blocks using defined chemical reactions with product validation. Use when creating combinatorial libraries or enumer...
Query protein-protein and gene interaction databases including STRING, BioGRID, and IntAct via their REST APIs and Python clients. Retrieve interaction networks, confidence scores, and functional enrichment. Use when building protein interaction netw...
End-to-end MeRIP-seq analysis from FASTQ to m6A peaks and differential methylation. Use when analyzing epitranscriptomic m6A modifications from immunoprecipitation data.
Detect transcription factor binding sites through footprinting analysis in ATAC-seq data using TOBIAS. Use when identifying TF occupancy patterns within accessible regions, as TF binding protects DNA from Tn5 cutting.
Quality control of phasing and imputation results. Filter by INFO scores, assess accuracy, and prepare imputed data for downstream analysis. Use when filtering low-quality imputed variants or validating imputation accuracy before GWAS.
End-to-end bisulfite sequencing workflow from FASTQ to differentially methylated regions. Covers Bismark alignment, methylation calling, and DMR detection with methylKit. Use when analyzing bisulfite sequencing data.
Deploy to edge runtimes (Cloudflare Workers, Vercel Edge, Deno Deploy) for globally distributed, low-latency applications. Master edge middleware, streaming, and runtime constraints for 2025+ edge computing.
Plan a new feature with analysis, design, and implementation steps. Use when the user asks to plan a feature or run /plan-feature.
Verify performance implications were considered and no obvious anti-patterns exist. Issues result in WARNINGS.
Checkpoint framework and validation rules for all agents. Ensures quality gates are passed at every stage of development.
Document software architecture using ARCHITECTURE.md and docs/*.md files with Mermaid diagrams. Use proactively when ARCHITECTURE.md exists in project root, or invoke to create initial architecture documentation. Covers system design, data flows, com...
Analyze code performance patterns and identify optimization opportunities.
Create, manage, and optimize virtual machines in Proxmox. Control VM lifecycle, monitor performance, adjust resources, and plan VM deployment strategies.
Auto-invoke when reviewing try/catch blocks, API error responses, async operations, or user feedback patterns. Enforces graceful degradation and meaningful error messages.
Test-Driven Development workflow - write tests first, then implementation
Techniques for analyzing existing codebases to reverse-engineer requirements and understand business logic. Use when conducting brownfield analysis or understanding existing system capabilities.
將提示詞和程式碼從 Claude Sonnet 4.0、Sonnet 4.5 或 Opus 4.1 遷移至 Opus 4.5。當使用者想要更新其程式碼庫、提示詞或 API 呼叫以使用 Opus 4.5 時使用。處理模型字串更新和針對已知 Opus 4.5 行為差異的提示詞調整。不會遷移 Haiku 4.5。
Extract coding standards and conventions from CONTRIBUTING.md, .editorconfig, linter configs. Use for onboarding and ensuring consistent contributions.
Create a thoughtful, supportive commit message from your staged changes.
Guides creation of high-quality MCP (Model Context Protocol) servers that enable LLMs to interact with external services through well-designed tools. Use when building MCP servers to integrate external APIs or services, whether in Python (FastMCP) or...
WordPress Playground for instant browser-based WordPress testing. Use for quick demos, plugin testing, or ephemeral development environments without Docker.
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