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End-to-end MeRIP-seq analysis from FASTQ to m6A peaks and differential methylation. Use when analyzing epitranscriptomic m6A modifications from immunoprecipitation data.
Quality control of phasing and imputation results. Filter by INFO scores, assess accuracy, and prepare imputed data for downstream analysis. Use when filtering low-quality imputed variants or validating imputation accuracy before GWAS.
Techniques for analyzing existing codebases to reverse-engineer requirements and understand business logic. Use when conducting brownfield analysis or understanding existing system capabilities.
Comprehensive knowledge about network reconnaissance and service enumeration. Provides methodologies for port scanning, service fingerprinting, web directory discovery, and vulnerability identification. Includes best practices for structured data col...
Guides creation of high-quality MCP (Model Context Protocol) servers that enable LLMs to interact with external services through well-designed tools. Use when building MCP servers to integrate external APIs or services, whether in Python (FastMCP) or...
Configure Claude Code sandbox network isolation with trusted domains, custom access policies, and environment variables for secure network communication.
Manage database schema changes with version control. Use when modifying DB schema, adding tables/columns, or setting up new projects. Covers Prisma, Drizzle, and migration best practices.
Pattern for aggregating insights across multiple tasks to enable data-driven evolution.
Use when setting up environment configuration, loading .env files, or managing application settings across environments. Triggers for: .env setup, loading environment variables, Pydantic BaseSettings, configuration validation, or secret management. N...
Checkpoint framework and validation rules for all agents. Ensures quality gates are passed at every stage of development.
將提示詞和程式碼從 Claude Sonnet 4.0、Sonnet 4.5 或 Opus 4.1 遷移至 Opus 4.5。當使用者想要更新其程式碼庫、提示詞或 API 呼叫以使用 Opus 4.5 時使用。處理模型字串更新和針對已知 Opus 4.5 行為差異的提示詞調整。不會遷移 Haiku 4.5。
Plan a new feature with analysis, design, and implementation steps. Use when the user asks to plan a feature or run /plan-feature.
Deploy to edge runtimes (Cloudflare Workers, Vercel Edge, Deno Deploy) for globally distributed, low-latency applications. Master edge middleware, streaming, and runtime constraints for 2025+ edge computing.
Expert at integrating Model Context Protocol (MCP) servers into Claude Code plugins. Auto-invokes when the user wants to add external tool integrations, configure MCP servers, set up stdio/SSE/HTTP/WebSocket connections, or needs help with MCP authen...
Verify performance implications were considered and no obvious anti-patterns exist. Issues result in WARNINGS.
This skill should be used when the user asks about "rust workspace", "rust best practices", "cargo workspace setup", "rust code organization", "rust dependency management", "rust testing strategy", "rust project", "scalable rust", "rust CI setup", or...
Guide for creating beautiful Mermaid diagrams with proper styling for GitHub markdown (dark/light mode compatible, no icons).
Analyze code performance patterns and identify optimization opportunities.
WordPress Playground for instant browser-based WordPress testing. Use for quick demos, plugin testing, or ephemeral development environments without Docker.
Direct REST API access to KEGG (academic use only). Pathway analysis, gene-pathway mapping, metabolic pathways, drug interactions, ID conversion. For Python workflows with multiple databases, prefer bioservices. Use this for direct HTTP/REST work or...
Code review of current git changes, compare to related plan if exists, identify bad engineering, over-engineering, or suboptimal solutions. Use when user asks to review changes, check git diff, validate implementation quality, or assess code changes.
Design tools that agents can use effectively. Use when creating new tools for agents, debugging tool-related failures, or optimizing existing tool sets.
Software Bill of Materials (SBOM) generation using Syft for container images, filesystems, and archives. Detects packages across 28+ ecosystems with multi-format output support (CycloneDX, SPDX, syft-json). Enables vulnerability assessment, license c...
Efficient database search tool for bioRxiv preprint server. Use this skill when searching for life sciences preprints by keywords, authors, date ranges, or categories, retrieving paper metadata, downloading PDFs, or conducting literature reviews.
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